Metagenomic and metatranscriptomic profiling of Lactobacillus casei Zhang in the human gut.

ABSTRACT: Little is known about the replication and dynamic transcription of probiotics during their "passenger" journey in the human GI tract, which has therefore limited the understanding of their probiotic mechanisms. Here, metagenomic and metatranscriptomic sequencing was used to expose the in vivo expression patterns of the probiotic Lactobacillus casei Zhang (LcZ), which was compared with its in vitro growth transcriptomes, as well as the dynamics of the indigenous microbiome response to probiotic consumption. Extraction of the strain-specific reads revealed that replication and transcripts from the ingested LcZ were increased, while those from the resident L. casei strains remained unchanged. Mapping of all sequencing reads to LcZ genome showed that gene expression in vitro and in vivo differ

SUBMITTER: Wang J 

PROVIDER: S-EPMC8249650 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature