Single-Target Regulators Constitute the Minority Group of Transcription Factors in Escherichia coli K-12.

ABSTRACT: The identification of regulatory targets of all transcription factors (TFs) is critical for understanding the entire network of genome regulation. A total of approximately 300 TFs exist in the model prokaryote Escherichia coli K-12, but the identification of whole sets of their direct targets is impossible with use of in vivo approaches. For this end, the most direct and quick approach is to identify the TF-binding sites in vitro on the genome. We then developed and utilized the gSELEX screening system in vitro for identification of more than 150 E. coli TF-binding sites along the E. coli genome. Based on the number of predicted regulatory targets, we classified E. coli K-12 TFs into four groups, altogether forming a hierarchy ranging from a

SUBMITTER: Shimada T 

PROVIDER: S-EPMC8249747 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature