The intact parasympathetic nerve promotes submandibular gland regeneration through ductal cell proliferation.

ABSTRACT:

Objectives

Salivary gland regeneration is closely related to the parasympathetic nerve; however, the mechanism behind this relationship is still unclear. The aim of this study was to evaluate the relationship between the parasympathetic nerve and morphological differences during salivary gland regeneration.

Materials and methods

We used a duct ligation/deligation-induced submandibular gland regeneration model of Sprague-Dawley (SD) rats. The regenerated submandibular gland with or without chorda lingual (CL) innervation was detected by haematoxylin-eosin staining, real-time PCR (RT-PCR), immunohistochemistry and Western blotting. We counted the number of Ki67-positive cells to reveal the proliferation process that occurs during gland regeneration. Finally, we examined the ex

SUBMITTER: Wang X 

PROVIDER: S-EPMC8249781 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature