Mass cytometry and transcriptomic profiling reveal body-wide pathology induced by Loxl1 deficiency.

ABSTRACT:

Objective

The loss of LOXL1 expression reportedly leads to the prolapse of pelvic organs or to exfoliation syndrome glaucoma. Increasing evidence suggests that LOXL1 deficiency is associated with the pathogenesis of several other diseases. However, the characterization of the systemic functions of LOXL1 is limited by the lack of relevant investigative technologies.

Materials and methods

To determine the functions of LOXL1, a novel method for body-wide organ transcriptome profiling, combined with single-cell mass cytometry, was developed. A body-wide organ transcriptomic (BOT) map was created by RNA-Seq of tissues from 17 organs from both Loxl1 knockout (KO) and wild-type mice.

Results

The BOT results indicated the systemic upregulation of genes encoding proteins assoc

SUBMITTER: Li Y 

PROVIDER: S-EPMC8249785 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature