Genome stabilization by RAD51-stimulatory compound 1 enhances efficiency of somatic cell nuclear transfer-mediated reprogramming and full-term development of cloned mouse embryos.

ABSTRACT:

Objectives

The genetic instability and DNA damage arise during transcription factor-mediated reprogramming of somatic cells, and its efficiency may be reduced due to abnormal chromatin remodelling. The efficiency in somatic cell nuclear transfer (SCNT)-mediated reprogramming is also very low, and it is caused by development arrest of most reconstituted embryos.

Materials and methods

Whether the repair of genetic instability or double-strand breaks (DSBs) during SCNT reprogramming may play an important role in embryonic development, we observed and analysed the effect of Rad 51, a key modulator of DNA damage response (DDR) in SCNT-derived embryos.

Results

Here, we observed that the activity of Rad 51 is lower in SCNT eggs than in conventional IVF and found a significan

SUBMITTER: Lee AR 

PROVIDER: S-EPMC8249786 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature