A novel mouse model for liver metastasis of prostate cancer reveals dynamic tumour-immune cell communication.

ABSTRACT:

Objectives

In contrast to extensive studies on bone metastasis in advanced prostate cancer (PCa), liver metastasis has been under-researched so far. In order to decipher molecular and cellular mechanisms underpinning liver metastasis of advanced PCa, we develop a rapid and immune sufficient mouse model for liver metastasis of PCa via orthotopic injection of organoids from PbCre⁺ ; rb1^f/f ;p53^f/f mice.

Materials and methods

PbCre⁺ ;rb1^f/f ;p53^f/f and PbCre⁺ ;pten^f/f ;p53^f/f mice were used to generate PCa organoid cultures in vitro. Immune sufficient liver metastasis models were established via orthotopic transplantation of organoids into the prostate of C57BL/6 mice. Immunofluor

SUBMITTER: Liu K 

PROVIDER: S-EPMC8249794 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature