The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells.

ABSTRACT:

Background

Zebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish, including RNAi, Cre/lox, and CRISPR-Cas9. However, the lac operator-repressor system, an E. coli lac operon component which has been adapted for use in many other species and is a valuable, flexible tool for inducible modulation of gene expression studies, has not been previously tested in zebrafish.

Results

Here we demonstrate that the lac operator-repressor system robustly decreases expression of firefly luciferase in cultured zebrafish fibroblast cells. Our work establishes the lac operator-repressor

SUBMITTER: Nishizaki SS 

PROVIDER: S-EPMC8249864 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature