Stepwise chromatin and transcriptional acquisition of an intraepithelial lymphocyte program.

ABSTRACT: Mesenteric lymph node (mLN) T cells undergo tissue adaptation upon migrating to intestinal lamina propria and epithelium, ensuring appropriate balance between tolerance and resistance. By combining mouse genetics with single-cell and chromatin analyses, we uncovered the molecular imprinting of gut epithelium on T cells. Transcriptionally, conventional and regulatory (T_reg) CD4⁺ T cells from mLN, lamina propria and intestinal epithelium segregate based on the gut layer they occupy; trajectory analysis suggests a stepwise loss of CD4 programming and acquisition of an intraepithelial profile. T_reg cell fate mapping coupled with RNA sequencing and assay for transposase-accessible chromatin followed by sequencing revealed that the T_reg cell program sh

SUBMITTER: London M 

PROVIDER: S-EPMC8251700 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature