Component-resolved diagnosis using guinea-pig allergens elucidates allergen sensitization profiles in allergy to furry animals.

ABSTRACT:

Background

Furry animals are an important source of indoor allergens. Diagnosis of allergy to small pets such as guinea-pigs still relies on animal dander extracts which do not allow to define the primary sensitization source.

Objective

To identify major guinea-pig allergens and to evaluate their potential as marker allergens for in vitro IgE-diagnosis in comparison with dander extracts.

Methods

A group of patients allergic to guinea-pig (n = 29) and a group of patients allergic to cat and dog (n = 30) were recruited for the study. A panel of four guinea-pig lipocalin allergens was expressed as recombinant proteins in E. coli. Specific IgE were quantified by ImmunoCAP and ELISA.

Results

The combination of 4 guinea-pig lipocalin allergens, including 2 new lipoca

SUBMITTER: Swiontek K 

PROVIDER: S-EPMC8251889 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature