Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector.

ABSTRACT: Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR-associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR-Cas strategies in plants rely on genetic transformation using Agrobacterium tumefaciens to supply the gene editing reagents, such as Cas nucleases or the synthetic guide RNA (sgRNA). While Cas nucleases are constant elements in editing approaches, sgRNAs are target-specific and a screening process is usually required to identify those most effective. Plant virus-derived vectors are an alternative for the fast and efficient delivery of sgRNAs into adult plants, due to the virus capacity for genome amplification and systemic movement, a strategy known as virus-induced genome editing. W

SUBMITTER: Uranga M 

PROVIDER: S-EPMC8251967 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature