PML-II regulates ERK and AKT signal activation and IFNα-induced cell death.

ABSTRACT:

Background

The requirement of promyelocytic leukaemia protein (PML) in interferon (IFN)-induced cell apoptosis is well-established. However, the exact mechanisms by which the multiple isoforms of PML protein participate in this process remain not well-understood. We previously demonstrated that PML isoform II (PML-II) positively regulates induced gene expression during a type I IFN response and evaluate here how PML-II contributes to IFNα-induced cell death.

Methods

HeLa cells were transiently depleted of PML-II by siRNA treatment and the response of these cells to treatment with IFNα assessed by molecular assays of mRNA and proteins associated with IFN and apoptosis responses.

Results

In HeLa cells, death during IFNα stimulation was reduced by prior PML-II depletion.

SUBMITTER: Meng X 

PROVIDER: S-EPMC8252201 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature