A modified protocol for successful miRNA profiling in human precision-cut lung slices (PCLS).

ABSTRACT:

Objective

Human precision cut lung slices (PCLS) are widely used as an ex vivo model system for drug discovery and development of new therapies. PCLS reflect the functional heterogeneity of lung tissue and possess relevant lung cell types. We thus determined the use of PCLS in studying non-coding RNAs notably miRNAs, which are important gene regulatory molecules. Since miRNAs play key role as mediators of respiratory diseases, they can serve as valuable prognostic or diagnostic biomarkers, and in therapeutic interventions, of lung diseases. A technical limitation though is the vast amount of agarose in PCLS which impedes (mi)RNA extraction by standard procedures. Here we modified our recently published protocol for RNA isolation from PCLS to enable miRNA readouts.

Results

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SUBMITTER: Niehof M 

PROVIDER: S-EPMC8252208 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature