Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study.

ABSTRACT:

Background

In biological cells, promoters drive gene expression by specific binding of RNA polymerase. They determine the starting position, timing and level of gene expression. Therefore, rational fine-tuning of promoters to regulate the expression levels of target genes for optimizing biosynthetic pathways in metabolic engineering has recently become an active area of research.

Results

In this study, we systematically detected and characterized the common promoter elements in the unconventional yeast Yarrowia lipolytica, and constructed an artificial hybrid promoter library that covers a wide range of promoter strength. The results indicate that the hybrid promoter strength can be fine-tuned by promoter elements, namely, upstream activation sequences (UAS), TATA box and co

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8252286 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature