Echocardiography-guided percutaneous left ventricular intracavitary injection as a cell delivery approach in infarcted mice.

ABSTRACT: In the field of cell therapy for heart disease, a new paradigm of repeated dosing of cells has recently emerged. However, the lack of a repeatable cell delivery method in preclinical studies in rodents is a major obstacle to investigating this paradigm. We have established and standardized a method of echocardiography-guided percutaneous left ventricular intracavitary injection (echo-guided LV injection) as a cell delivery approach in infarcted mice. Here, we describe the method in detail and address several important issues regarding it. First, by integrating anatomical and echocardiographic considerations, we have established strategies to determine a safe anatomical window for injection in infarcted mice. Second, we summarize our experience with this method (734 injections). The overall

SUBMITTER: Nong Y 

PROVIDER: S-EPMC8252912 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature