High-sensitivity SARS-CoV-2 group testing by digital PCR among symptomatic patients in hospital settings.

ABSTRACT:

Background

Worldwide demand for SARS-CoV-2 RT-PCR testing is still high as testing remains central to follow the disease spread and vaccine efficacy. Group testing has been proposed as a solution to expand testing capabilities but sensitivity concerns may limit its impact on the management of the pandemic. Digital PCR (RT-dPCR) has been shown to be highly sensitive and could help by providing larger testing capabilities without compromising sensitivity.

Methods

We implemented RT-dPCR based COVID-19 group testing on a commercially available system and assay (naica® system from Stilla Technologies) and investigated the sensitivity of the method in real life conditions of a university hospital in Paris, France, in May 2020. We tested the protocol in a direct comparison with ref

SUBMITTER: Martin A 

PROVIDER: S-EPMC8253657 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature