A mass spectrometry-based targeted assay for detection of SARS-CoV-2 antigen from clinical specimens.

ABSTRACT:

Background

The COVID-19 pandemic caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has overwhelmed health systems worldwide and highlighted limitations of diagnostic testing. Several types of diagnostic tests including RT-PCR-based assays and antigen detection by lateral flow assays, each with their own strengths and weaknesses, have been developed and deployed in a short time.

Methods

Here, we describe an immunoaffinity purification approach followed a by high resolution mass spectrometry-based targeted qualitative assay capable of detecting SARS-CoV-2 viral antigen from nasopharyngeal swab samples. Based on our discovery experiments using purified virus, recombinant viral protein and nasopharyngeal swab samples from COVID-19 positive patients, nucleoca

SUBMITTER: Renuse S 

PROVIDER: S-EPMC8253671 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature