Interlaboratory study for the evaluation of three microtiter plate-based biofilm quantification methods.

ABSTRACT: Microtiter plate methods are commonly used for biofilm assessment. However, results obtained with these methods have often been difficult to reproduce. Hence, it is important to obtain a better understanding of the repeatability and reproducibility of these methods. An interlaboratory study was performed in five different laboratories to evaluate the reproducibility and responsiveness of three methods to quantify Staphylococcus aureus biofilm formation in 96-well microtiter plates: crystal violet, resazurin, and plate counts. An inter-lab protocol was developed for the study. The protocol was separated into three steps: biofilm growth, biofilm challenge, biofilm assessment. For control experiments participants performed the growth and assessment steps only. For treatment experiments, all t

SUBMITTER: Allkja J 

PROVIDER: S-EPMC8253847 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature