Glucose treatment of human pancreatic β-cells enhances translation of mRNAs involved in energetics and insulin secretion.

ABSTRACT: Glucose-mediated signaling regulates the expression of a limited number of genes in human pancreatic β-cells at the transcriptional level. However, it is unclear whether glucose plays a role in posttranscriptional RNA processing or translational control of gene expression. Here, we asked whether glucose affects posttranscriptional steps and regulates protein synthesis in human β-cell lines. We first showed the involvement of the mTOR pathway in glucose-related signaling. We also used the surface sensing of translation technique, based on puromycin incorporation into newly translated proteins, to demonstrate that glucose treatment increased protein translation. Among the list of glucose-induced proteins, we identified the proconvertase PCSK1, an enzyme involved in the proteolytic conversion

SUBMITTER: Zakaria A 

PROVIDER: S-EPMC8253965 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature