Chromosomal analyses of human giant diploid oocytes by next-generation sequencing.

ABSTRACT:

Purpose

Although giant oocytes (GOs) having about twice cytoplasmic volume compared with general oocytes in mammals including the human are rarely recovered, it is thought that GOs have potentially chromosomal abnormalities. The aim of the present study was to assess chromosome numbers in chromosome-spindle complexes (CSCs) and polar bodies of human GOs by using micromanipulation for sampling and next-generation sequencing (NGS) for analyses of the chromosome numbers.

Methods

When recovered oocytes whose cytoplasm has lager than 140 µm or above, the oocytes were defined as GOs, and recovered GOs were vitrified. After warming, the CSCs, polar bodies, and enucleated cytoplasm were collected by micromanipulation from 3 GOs. The collected samples were analyzed by NGS.

Results

SUBMITTER: Kawano H 

PROVIDER: S-EPMC8254173 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature