Inter-study and time-dependent variability of metabolite abundance in cultured red blood cells.

ABSTRACT:

Background

Cultured human red blood cells (RBCs) provide a powerful ex vivo assay platform to study blood-stage malaria infection and propagation. In recent years, high-resolution metabolomic methods have quantified hundreds of metabolites from parasite-infected RBC cultures under a variety of perturbations. In this context, the corresponding control samples of the uninfected culture systems can also be used to examine the effects of these perturbations on RBC metabolism itself and their dependence on blood donors (inter-study variations).

Methods

Time-course datasets from five independent studies were generated and analysed, maintaining uninfected RBCs (uRBC) at 2% haematocrit for 48 h under conditions originally designed for parasite cultures. Using identical experimental

SUBMITTER: Tewari SG 

PROVIDER: S-EPMC8254254 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature