Hypoproliferative human neural progenitor cell xenografts survived extendedly in the brain of immunocompetent rats.

ABSTRACT:

Background

There is a huge controversy about whether xenograft or allograft in the "immune-privileged" brain needs immunosuppression. In animal studies, the prevailing sophisticated use of immunosuppression or immunodeficient animal is detrimental for the recipients, which results in a short lifespan of animals, confounds functional behavioral readout of the graft benefits, and discourages long-term follow-up.

Methods

Neuron-restricted neural progenitor cells (NPCs) were derived from human embryonic stem cells (ESCs, including H1, its gene-modified cell lines for better visualization, and HN4), propagated for different passages, and then transplanted into the brain of immunocompetent rats without immunosuppressants. The graft survivals, their cell fates, and HLA expression l

SUBMITTER: Liu C 

PROVIDER: S-EPMC8254296 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature