Pre-processing tissue specimens with a tissue homogenizer: clinical and microbiological evaluation.

ABSTRACT:

Background

Tissues are valuable specimens in diagnostic microbiology because they are often obtained by invasive methods, and effort should thus be taken to maximize microbiological yield. The objective of this study was to evaluate the added value of using tissue pre-processing (tissue homogenizer instrument gentleMACS Dissociator) in detecting microorganisms responsible for infections.

Methods

We included 104 randomly collected tissue samples, 41 (39.4 %) bones and 63 (60.6 %) soft tissues, many of those (42/104 (40.4 %)) were of periprosthetic origins. We compared the agreement between pre-processing tissues using tissue homogenizer with routine microbiology diagnostic procedure, and we calculated the performance of these methods when clinical infections were used as refe

SUBMITTER: Yusuf E 

PROVIDER: S-EPMC8254327 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature