A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum.

ABSTRACT:

Background

CRISPR/Cas9 is a rapidly developing genome editing technology in various biological systems due to its efficiency, portability, simplicity and versatility. This editing technology has been successfully applied in in several important plants of Solanaceae such as tomato, tobacco, potato, petunia and groundcherry. Wolfberry ranked the sixth among solanaceous crops of outstanding importance in China following potato, tomato, eggplant, pepper and tobacco. To date, there has been no report on CRISPR/Cas9 technology to improve Lycium ruthenicum due to the unknown genome sequencing and the lack of efficient regeneration and genetic transformation systems.

Results

In this study, we have established an efficientregeneration and genetic transformation system of Lycium ruthe

SUBMITTER: Wang W 

PROVIDER: S-EPMC8254353 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature