Large-scale analysis of the position-dependent binding and regulation of human RNA binding proteins.

ABSTRACT:

Background

RNA binding proteins (RBPs) play essential roles in the regulation of RNA metabolism. Recent studies have disclosed that RBPs achieve their functions via binding to their targets in a position-dependent pattern on RNAs. However, few studies have systematically addressed the associations between the RBP's functions and their positional binding preferences.

Methods

Here, we present large-scale analyses on the functional targets of human RBPs by integrating the enhanced cross-linking and immunoprecipitation followed by sequencing (eCLIP-seq) datasets and the shRNA knockdown followed by RNA-seq datasets that are deposited in the integrated ENCyclopedia of DNA Elements in the human genome (ENCODE) data portal.

Results

We found that (1) binding to the translation

SUBMITTER: Lin J 

PROVIDER: S-EPMC8254518 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature