Biotransformation of hydroquinone into α-arbutin by transglucosylation activity of a metagenomic amylosucrase.

ABSTRACT: Arbutin is a naturally occurring glycosylated product of hydroquinone. With the ability to interrupt melanin biosynthesis in epidermal cells, it is a promising cosmetic ingredient. In this study, a novel amylosucrase, As_met, identified from a thermal spring metagenome, has been characterized for arbutin biosynthesis. As_met was able to catalyze transglucosylation of hydroquinone to arbutin, taking sucrose as glycosyl donor, in the temperature range of 20 °C to 40 °C and pH 5.0 to 6.0, with the relative activity of 80% or more. The presence of chloride salts of Li, K, and Na at 1 mM concentration did not exhibit any notable effect on the enzyme's activity, unlike Cu, Ni, and Mn, which were observed to be detrimental. The hydroquinone (20 mM) to sucrose ratio of 1:1 to 1

SUBMITTER: Agarwal N 

PROVIDER: S-EPMC8254693 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature