A study of the composition of the Obsoletus complex and genetic diversity of Culicoides obsoletus populations in Spain.

ABSTRACT:

Background

The Culicoides obsoletus species complex (henceforth 'Obsoletus complex') is implicated in the transmission of several arboviruses that can cause severe disease in livestock, such as bluetongue, African horse sickness, epizootic hemorrhagic disease and Schmallenberg disease. Thus, this study aimed to increase our knowledge of the composition and genetic diversity of the Obsoletus complex by partial sequencing of the cytochrome c oxidase I (cox1) gene in poorly studied areas of Spain.

Methods

A study of C. obsoletus populations was carried out using a single-tube multiplex polymerase chain reaction (PCR) assay that was designed to differentiate the Obsoletus complex sibling species Culicoides obsoletus and Culicoides scoticus, based on the partial amplification of

SUBMITTER: Aguilar-Vega C 

PROVIDER: S-EPMC8254917 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature