GRINS: Genetic elements that recode assembly-line polyketide synthases and accelerate their diversification.

ABSTRACT: Assembly-line polyketide synthases (PKSs) are large and complex enzymatic machineries with a multimodular architecture, typically encoded in bacterial genomes by biosynthetic gene clusters. Their modularity has led to an astounding diversity of biosynthesized molecules, many with medical relevance. Thus, understanding the mechanisms that drive PKS evolution is fundamental for both functional prediction of natural PKSs as well as for the engineering of novel PKSs. Here, we describe a repetitive genetic element in assembly-line PKS genes which appears to play a role in accelerating the diversification of closely related biosynthetic clusters. We named this element GRINS: genetic repeats of intense nucleotide skews. GRINS appear to recode PKS protein regions with a biased nucleotide compositi

SUBMITTER: Nivina A 

PROVIDER: S-EPMC8256042 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature