Piperacillin triggers virulence factor biosynthesis via the oxidative stress response in Burkholderia thailandensis.

ABSTRACT: Natural products have been an important source of therapeutic agents and chemical tools. The recent realization that many natural product biosynthetic genes are silent or sparingly expressed during standard laboratory growth has prompted efforts to investigate their regulation and develop methods to induce their expression. Because it is difficult to intuit signals that induce a given biosynthetic locus, we recently implemented a forward chemical-genetic approach to identify such inducers. In the current work, we applied this approach to nine silent biosynthetic loci in the model bacterium Burkholderia thailandensis to systematically screen for elicitors from a library of Food and Drug Administration-approved drugs. We find that β-lactams, fluoroquinolones, antifungals, and, surprisingly,

SUBMITTER: Li A 

PROVIDER: S-EPMC8256049 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature