Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses.

ABSTRACT: Super-resolution imaging has revealed that key synaptic proteins are dynamically organized within sub-synaptic domains (SSDs). To examine how different inhibitory receptors are regulated, we carried out dual-color direct stochastic optical reconstruction microscopy (dSTORM) of GlyRs and GABA_A Rs at mixed inhibitory synapses in spinal cord neurons. We show that endogenous GlyRs and GABA_A Rs as well as their common scaffold protein gephyrin form SSDs that align with pre-synaptic RIM1/2, thus creating trans-synaptic nanocolumns. Strikingly, GlyRs and GABA_A Rs occupy different sub-synaptic spaces, exhibiting only a partial overlap at mixed inhibitory synapses. When network activity is increased by 4-aminopyridine treatment, the GABA_A R copy numbers a

SUBMITTER: Yang X 

PROVIDER: S-EPMC8256292 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature