Rapid ordering of barcoded transposon insertion libraries of anaerobic bacteria.

ABSTRACT: Commensal bacteria from the human intestinal microbiota play important roles in health and disease. Research into the mechanisms by which these bacteria exert their effects is hampered by the complexity of the microbiota, the strict growth requirements of the individual species and a lack of genetic tools and resources. The assembly of ordered transposon insertion libraries, in which nearly all nonessential genes have been disrupted and the strains stored as independent monocultures, would be a transformative resource for research into many microbiota members. However, assembly of these libraries must be fast and inexpensive in order to empower investigation of the large number of species that typically compose gut communities. The methods used to generate ordered libraries must also be ad

SUBMITTER: Shiver AL 

PROVIDER: S-EPMC8256429 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature