Artificial complementary chromatic acclimation gene expression system in Escherichia coli.

ABSTRACT:

Background

The development of multiple gene expression systems, especially those based on the physical signals, such as multiple color light irradiations, is challenging. Complementary chromatic acclimation (CCA), a photoreversible process that facilitates the control of cellular expression using light of different wavelengths in cyanobacteria, is one example. In this study, an artificial CCA systems, inspired by type III CCA light-regulated gene expression, was designed by employing a single photosensor system, the CcaS/CcaR green light gene expression system derived from Synechocystis sp. PCC6803, combined with G-box (the regulator recognized by activated CcaR), the cognate cpcG2 promoter, and the constitutively transcribed promoter, the P_trcΔLacO promoter.

Results<

SUBMITTER: Ariyanti D 

PROVIDER: S-EPMC8256508 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature