Plant super-barcode: a case study on genome-based identification for closely related species of Fritillaria.

ABSTRACT:

Background

Although molecular analysis offers a wide range of options for species identification, a universal methodology for classifying and distinguishing closely related species remains elusive. This study validated the effectiveness of utilizing the entire chloroplast (cp) genome as a super-barcode to help identify and classify closely related species.

Methods

We here compared 26 complete cp genomes of ten Fritillaria species including 18 new sequences sequenced in this study. Each species had repeats and the cp genomes were used as a whole DNA barcode to test whether they can distinguish Fritillaria species.

Results

The cp genomes of Fritillaria medicinal plants were conserved in genome structure, gene type, and gene content. Comparison analysis of the Fritillari

SUBMITTER: Wu L 

PROVIDER: S-EPMC8256587 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature