Fixed-target serial femtosecond crystallography using <i>in cellulo</i> grown microcrystals.

ABSTRACT: The crystallization of recombinant proteins in living cells is an exciting new approach in structural biology. Recent success has highlighted the need for fast and efficient diffraction data collection, optimally directly exposing intact crystal-containing cells to the X-ray beam, thus protecting the in cellulo crystals from environmental challenges. Serial femtosecond crystallography (SFX) at free-electron lasers (XFELs) allows the collection of detectable diffraction even from tiny protein crystals, but requires very fast sample exchange to utilize each XFEL pulse. Here, an efficient approach is presented for high-resolution structure elucidation using serial femtosecond in cellulo diffraction of micometre-sized crystals of the protein HEX-1 from the fungus Neurospora cr

SUBMITTER: Lahey-Rudolph JM 

PROVIDER: S-EPMC8256716 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature