A Bayesian inference transcription factor activity model for the analysis of single-cell transcriptomes.

ABSTRACT: Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful experimental approach to study cellular heterogeneity. One of the challenges in scRNA-seq data analysis is integrating different types of biological data to consistently recognize discrete biological functions and regulatory mechanisms of cells, such as transcription factor activities and gene regulatory networks in distinct cell populations. We have developed an approach to infer transcription factor activities from scRNA-seq data that leverages existing biological data on transcription factor binding sites. The Bayesian inference transcription factor activity model (BITFAM) integrates ChIP-seq transcription factor binding information into scRNA-seq data analysis. We show that the inferred transcription factor activities for

SUBMITTER: Gao S 

PROVIDER: S-EPMC8256867 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature