Splicing and editing of ionotropic glutamate receptors: a comprehensive analysis based on human RNA-Seq data.

ABSTRACT: Ionotropic glutamate receptors (iGluRs) play key roles for signaling in the central nervous system. Alternative splicing and RNA editing are well-known mechanisms to increase iGluR diversity and to provide context-dependent regulation. Earlier work on isoform identification has focused on the analysis of cloned transcripts, mostly from rodents. We here set out to obtain a systematic overview of iGluR splicing and editing in human brain based on RNA-Seq data. Using data from two large-scale transcriptome studies, we established a workflow for the de novo identification and quantification of alternative splice and editing events. We detected all canonical iGluR splice junctions, assessed the abundance of alternative events described in the literature, and identified new splice events in AMPA

SUBMITTER: Herbrechter R 

PROVIDER: S-EPMC8257547 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature