Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator.
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ABSTRACT:
SUBMITTER: MacDonald IC
PROVIDER: S-EPMC8257575 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Publications
Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator.
MacDonald I Cody IC Seamons Travis R TR Emmons Jonathan C JC Javdan Shwan B SB Deans Tara L TL
Nature communications 20210705 1
Expanding the genetic toolbox for prokaryotic synthetic biology is a promising strategy for enhancing the dynamic range of gene expression and enabling new engineered applications for research and biomedicine. Here, we reverse the current trend of moving genetic parts from prokaryotes to eukaryotes and demonstrate that the activating eukaryotic transcription factor QF and its corresponding DNA-binding sequence can be moved to E. coli to introduce transcriptional activation, in addition to tight ...[more]
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