Micro-aerobic production of isobutanol with engineered Pseudomonas putida.

ABSTRACT: Pseudomonas putida KT2440 is emerging as a promising microbial host for biotechnological industry due to its broad range of substrate affinity and resilience to physicochemical stresses. Its natural tolerance towards aromatics and solvents qualifies this versatile microbe as promising candidate to produce next generation biofuels such as isobutanol. In this study, we scaled-up the production of isobutanol with P. putida from shake flask to fed-batch cultivation in a 30 L bioreactor. The design of a two-stage bioprocess with separated growth and production resulted in 3.35 g_isobutanol L^-1. Flux analysis revealed that the NADPH expensive formation of isobutanol exceeded the cellular catabolic supply of NADPH finally causing growth retardation. Concomitantl

SUBMITTER: Ankenbauer A 

PROVIDER: S-EPMC8258000 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature