Integrated in silico MS-based phosphoproteomics and network enrichment analysis of RASopathy proteins.

ABSTRACT:

Background

RASopathies are a group of syndromes showing clinical overlap caused by mutations in genes affecting the RAS-MAPK pathway. Consequent disruption on cellular signaling leads and is driven by phosphoproteome remodeling. However, we still lack a comprehensive picture of the different key players and altered downstream effectors.

Methods

An in silico interactome of RASopathy proteins was generated using pathway enrichment analysis/STRING tool, including identification of main hub proteins. We also integrated phosphoproteomic and immunoblotting studies using previous published information on RASopathy proteins and their neighbors in the context of RASopathy syndromes. Data from Phosphosite database ( www.phosphosite.org ) was collected in order to obtain the potential

SUBMITTER: Montero-Bullon JF 

PROVIDER: S-EPMC8258961 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature