Traumatic brain injury results in unique microglial and astrocyte transcriptomes enriched for type I interferon response.

ABSTRACT:

Background

Traumatic brain injury (TBI) is a leading cause of death and disability that lacks neuroprotective therapies. Following a TBI, secondary injury response pathways are activated and contribute to ongoing neurodegeneration. Microglia and astrocytes are critical neuroimmune modulators with early and persistent reactivity following a TBI. Although histologic glial reactivity is well established, a precise understanding of microglia and astrocyte function following trauma remains unknown.

Methods

Adult male C57BL/6J mice underwent either fluid percussion or sham injury. RNA sequencing of concurrently isolated microglia and astrocytes was conducted 7 days post-injury to evaluate cell-type-specific transcriptional responses to TBI. Dual in situ hybridization and immunoflu

SUBMITTER: Todd BP 

PROVIDER: S-EPMC8259035 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature