Stress tolerance enhancement via SPT15 base editing in Saccharomyces cerevisiae.

ABSTRACT:

Background

Saccharomyces cerevisiae is widely used in traditional brewing and modern fermentation industries to produce biofuels, chemicals and other bioproducts, but challenged by various harsh industrial conditions, such as hyperosmotic, thermal and ethanol stresses. Thus, its stress tolerance enhancement has been attracting broad interests. Recently, CRISPR/Cas-based genome editing technology offers unprecedented tools to explore genetic modifications and performance improvement of S. cerevisiae.

Results

Here, we presented that the Target-AID (activation-induced cytidine deaminase) base editor of enabling C-to-T substitutions could be harnessed to generate in situ nucleotide changes on the S. cerevisiae genome, thereby introducing protein point mutations in cells. The gen

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8259078 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature