High-Speed Lens-Free Holographic Sensing of Protein Molecules Using Quantitative Agglutination Assays.

ABSTRACT: Accurate, cost-effective, easy-to-use, and point-of-care sensors for protein biomarker levels are important for disease diagnostics. A cost-effective and compact readout approach that has been used for several diagnostic applications is lens-free holographic microscopy, which provides an ultralarge field of view and submicron resolution when it is coupled with pixel super-resolution techniques. Despite its potential as a diagnostic technique, lens-free microscopy has not previously been applied to quantitative protein molecule sensing in solution, which can simplify sensing protocols and ultimately enable measurements of binding kinetics in physiological conditions. Here, we sense interferon-γ (an immune system biomarker) and NeutrAvidin molecules in solution by combining lens-free microsc

SUBMITTER: Xiong Z 

PROVIDER: S-EPMC8259606 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature