Enhancing Open Modification Searches via a Combined Approach Facilitated by Ursgal.

ABSTRACT: The identification of peptide sequences and their post-translational modifications (PTMs) is a crucial step in the analysis of bottom-up proteomics data. The recent development of open modification search (OMS) engines allows virtually all PTMs to be searched for. This not only increases the number of spectra that can be matched to peptides but also greatly advances the understanding of the biological roles of PTMs through the identification, and the thereby facilitated quantification, of peptidoforms (peptide sequences and their potential PTMs). Whereas the benefits of combining results from multiple protein database search engines have been previously established, similar approaches for OMS results have been missing so far. Here we compare and combine results from three different OMS eng

SUBMITTER: Schulze S 

PROVIDER: S-EPMC8259620 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature