Dysregulation of Principal Cell miRNAs Facilitates Epigenetic Regulation of AQP2 and Results in Nephrogenic Diabetes Insipidus.

ABSTRACT:

Background

MicroRNAs (miRNAs), formed by cleavage of pre-microRNA by the endoribonuclease Dicer, are critical modulators of cell function by post-transcriptionally regulating gene expression.

Methods

Selective ablation of Dicer in AQP2-expressing cells (Dicer^AQP2Cre+ mice) was used to investigate the role of miRNAs in the kidney collecting duct of mice.

Results

The mice had severe polyuria and nephrogenic diabetes insipidus, potentially due to greatly reduced AQP2 and AQP4 levels. Although epithelial sodium channel levels were decreased in cortex and increased in inner medulla, amiloride-sensitive sodium reabsorption was equivalent in Dicer^AQP2Cre+ mice and controls. Small-RNA sequencing and proteomic analysis revealed 31 and 178 significantly re

SUBMITTER: Petrillo F 

PROVIDER: S-EPMC8259636 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature