Crumbs2 Is an Essential Slit Diaphragm Protein of the Renal Filtration Barrier.

ABSTRACT:

Background

Crumbs2 is expressed at embryonic stages as well as in the retina, brain, and glomerular podocytes. Recent studies identified CRB2 mutations as a novel cause of steroid-resistant nephrotic syndrome (SRNS).

Methods

To study the function of Crb2 at the renal filtration barrier, mice lacking Crb2 exclusively in podocytes were generated. Gene expression and histologic studies as well as transmission and scanning electron microscopy were used to analyze these Crb2^podKO knockout mice and their littermate controls. Furthermore, high-resolution expansion microscopy was used to investigate Crb2 distribution in murine glomeruli. For pull-down experiments, live cell imaging, and transcriptome analyses, cell lines were applied that inducibly express f

SUBMITTER: Moller-Kerutt A 

PROVIDER: S-EPMC8259666 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature