Comparing Approaches to Normalize, Quantify, and Characterize Urinary Extracellular Vesicles.

ABSTRACT:

Background

Urinary extracellular vesicles (uEVs) are a promising source for biomarker discovery, but optimal approaches for normalization, quantification, and characterization in spot urines are unclear.

Methods

Urine samples were analyzed in a water-loading study, from healthy subjects and patients with kidney disease. Urine particles were quantified in whole urine using nanoparticle tracking analysis (NTA), time-resolved fluorescence immunoassay (TR-FIA), and EVQuant, a novel method quantifying particles via gel immobilization.

Results

Urine particle and creatinine concentrations were highly correlated in the water-loading study (R² 0.96) and in random spot urines from healthy subjects (R² 0.47-0.95) and patie

SUBMITTER: Blijdorp CJ 

PROVIDER: S-EPMC8259679 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature