Regulation of the gonadal transcriptome during sex determination and testis morphogenesis: comparative candidate genes.

ABSTRACT: Gene expression profiles during sex determination and gonadal differentiation were investigated to identify new potential regulatory factors. Embryonic day 13 (E13), E14, and E16 rat testes and ovaries were used for microarray analysis, as well as E13 testis organ cultures that undergo testis morphogenesis and develop seminiferous cords in vitro. A list of 109 genes resulted from a selective analysis for genes present in male gonadal development and with a 1.5-fold change in expression between E13 and E16. Characterization of these 109 genes potentially important for testis development revealed that cytoskeletal-associated proteins, extracellular matrix factors, and signaling factors were highly represented. Throughout the developmental period (E13-E16), sex-enriched transcripts were more

SUBMITTER: Clement TM 

PROVIDER: S-EPMC8260008 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature