Sensitivity assessment of workflows detecting rare circulating cell-free DNA targets: A study design proposal.

ABSTRACT: The field of liquid biopsy has seen extensive growth in recent decades, making it one of the most promising areas in molecular diagnostics. Circulating cell-free DNA (ccfDNA) especially is used as an analyte in a growing number of diagnostic assays. These assays require specified preanalytical workflows delivering ccfDNA in qualities and quantities that facilitate correct and reliable results. As each step and component used in the preanalytical process has the potential to influence the assay sensitivity and other performance characteristics, it is key to find an unbiased experimental setup to test these factors in diagnostic or research laboratories. We defined one such setup by using blood from healthy subjects and commercially available products for blood collection, spike-in material,

SUBMITTER: Voss T 

PROVIDER: S-EPMC8260181 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature