Local and CNS-Wide Astrocyte Intracellular Calcium Signaling Attenuation In Vivo with CalEx^flox Mice.

ABSTRACT: Astrocytes exist throughout the CNS and affect neural circuits and behavior through intracellular Ca²⁺ signaling. Studying the function(s) of astrocyte Ca²⁺ signaling has proven difficult because of the paucity of tools to achieve selective attenuation. Based on recent studies, we generated and used male and female knock-in mice for Cre-dependent expression of mCherry-tagged hPMCA2w/b to attenuate astrocyte Ca²⁺ signaling in genetically defined cells in vivo (CalEx^flox mice for Calcium Extrusion). We characterized CalEx^flox mice following local AAV-Cre microinjections into the striatum and found reduced astrocyte Ca²⁺ signaling (∼90%) accompanied with repetitive self-grooming behavior. We also crossed Ca

SUBMITTER: Yu X 

PROVIDER: S-EPMC8260243 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature