Characterization of functional protein complexes from Alzheimer's disease and healthy brain by mass spectrometry-based proteome analysis.

ABSTRACT: Alzheimer's disease (AD) is a complex neurodegenerative disorder with impaired protein activities. Proteins in the form of complexes have a ubiquitous role in diverse range of cellular functions. The key challenge is to identify novel disease associated protein complexes and their potential role in the progression of AD pathology. Protein complexes were obtained from AD brain prefrontal cortex and age matched controls by Blue Native-Polyacrylamide Gel Electrophoresis. A proteomic analysis was performed using second dimension SDS-PAGE followed by nano LC-MS/MS. Differentially expressed proteins were mapped to existing biological networks by Ingenuity Pathway Analysis (IPA). A total of 13 protein complexes with their interacting proteins were resolved on SDS-PAGE. We identified 34 protein sp

SUBMITTER: Hasan B 

PROVIDER: S-EPMC8260596 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature