Nigella sativa callus treated with sodium azide exhibit augmented antioxidant activity and DNA damage inhibition.

ABSTRACT: Nigella sativa L. (NS) is an herbaceous plant, possessing phytochemicals of therapeutic importance. Thymoquinone is one of the active phytochemicals of NS that confers noteworthy antioxidant properties. Sodium azide, an agent of abiotic stress, can modulates antioxidant system in plants. In the present investigation, sodium azide (0, 5 µM, 10 µM, 20 µM, 50 µM, 100 µM and 200 µM) doses administered to the in vitro NS callus cultures for production/modification of secondary metabolites with augmented activity. 200 µM sodium azide treated NS callus exhibited maximum peroxidase activity (1.286 ± 0.101 nanokatal mg^-1 protein) and polyphenol oxidase activity (1.590 ± 0.110 nanokatal mg^-1 protein), while 100 µM sodium azide treated NS callus for optimum catalase activity (1.

SUBMITTER: Iqbal MS 

PROVIDER: S-EPMC8260798 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature